Application Note

Optimization Of A Hydrophobic Interaction Chromatography Step For Recombinant Protein Purification

Source: Cytiva

By Eva Heldin, BioProcess R&D Section Leader, Cytiva

iStock-827581062-lab-computer

Explore a workflow employed for the purification of a recombinant protein expressed in E. coli utilizing hydrophobic interaction chromatography (HIC). This workflow includes the use of high-throughput process development (HTPD) plates to facilitate the choice of resin and initial conditions for column chromatography. The range of buffer conditions utilized in this study was based on the results of an initial solubility study, and all the resins used were Capto™ high productivity resins. Based on the chromatographic results, we found that a wash step before the elution step reduced the amount of impurities. We also performed a design of experiments (DoE) to optimize the wash step and the results were confirmed in a verification run.

access the Application Note!

Get unlimited access to:

Trend and Thought Leadership Articles
Case Studies & White Papers
Extensive Product Database
Members-Only Premium Content
Welcome Back! Please Log In to Continue. X

Enter your credentials below to log in. Not yet a member of Outsourced Pharma? Subscribe today.

Subscribe to Outsourced Pharma X

Please enter your email address and create a password to access the full content, Or log in to your account to continue.

or

Subscribe to Outsourced Pharma